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GenScript corporation cdna of gw1δ1a mutant
Cdna Of Gw1δ1a Mutant, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+of+gw1%CE%B41a+mutant/cdna+of+gw1%CE%B41a+mutant/pmc02673069-247-3-20
Average 90 stars, based on 1 article reviews
cdna of gw1δ1a mutant - by Bioz Stars, 2026-09
90/100 stars

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Mutagenesis:

Article Title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing
Article Snippet: To construct pENTR-GW1Δ10 (amino acids 566–1343), phrGFP-KIAA1460 was digested with XhoI (5′ end linker) and SmaI to release a 2.3-kb fragment, which was then subcloned into the SalI and EcoRV sites of pENTR2B (Invitrogen). .. The cDNA of GW1Δ1a mutant (tryptophan > alanine, W > A) was directly synthesized and subcloned in pUC57 vector by GenScript using the sequence as shown in the Supplemental Materials and Methods. .. The mutated gene sequence was moved to pDONR207 vector (Invitrogen) using Gateway BP recombination for further applications.

Synthesized:

Article Title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing
Article Snippet: To construct pENTR-GW1Δ10 (amino acids 566–1343), phrGFP-KIAA1460 was digested with XhoI (5′ end linker) and SmaI to release a 2.3-kb fragment, which was then subcloned into the SalI and EcoRV sites of pENTR2B (Invitrogen). .. The cDNA of GW1Δ1a mutant (tryptophan > alanine, W > A) was directly synthesized and subcloned in pUC57 vector by GenScript using the sequence as shown in the Supplemental Materials and Methods. .. The mutated gene sequence was moved to pDONR207 vector (Invitrogen) using Gateway BP recombination for further applications.

Plasmid Preparation:

Article Title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing
Article Snippet: To construct pENTR-GW1Δ10 (amino acids 566–1343), phrGFP-KIAA1460 was digested with XhoI (5′ end linker) and SmaI to release a 2.3-kb fragment, which was then subcloned into the SalI and EcoRV sites of pENTR2B (Invitrogen). .. The cDNA of GW1Δ1a mutant (tryptophan > alanine, W > A) was directly synthesized and subcloned in pUC57 vector by GenScript using the sequence as shown in the Supplemental Materials and Methods. .. The mutated gene sequence was moved to pDONR207 vector (Invitrogen) using Gateway BP recombination for further applications.

Sequencing:

Article Title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing
Article Snippet: To construct pENTR-GW1Δ10 (amino acids 566–1343), phrGFP-KIAA1460 was digested with XhoI (5′ end linker) and SmaI to release a 2.3-kb fragment, which was then subcloned into the SalI and EcoRV sites of pENTR2B (Invitrogen). .. The cDNA of GW1Δ1a mutant (tryptophan > alanine, W > A) was directly synthesized and subcloned in pUC57 vector by GenScript using the sequence as shown in the Supplemental Materials and Methods. .. The mutated gene sequence was moved to pDONR207 vector (Invitrogen) using Gateway BP recombination for further applications.



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GenScript corporation cdna of gw1δ1a mutant
Cdna Of Gw1δ1a Mutant, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+of+gw1%CE%B41a+mutant/cdna+of+gw1%CE%B41a+mutant/pmc02673069-247-3-20
Average 90 stars, based on 1 article reviews
cdna of gw1δ1a mutant - by Bioz Stars, 2026-09
90/100 stars
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